A total of three human samples and ten swine samples were PCR-positive and confirmed by sequencing.

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Vietnamese VN strains are colored in grey, Malawian VS5700009 in light yellow and Tunisian TN25 and TN18 appear in orange Statistical support of grouping from Bayesian posterior probabilities (clade credibilities ≥90%) and 1,000 parallel Maximum Likelihood bootstrap replicates (≥70%) is indicated at the nodes. The authors declare no competing financial interests. This work is licensed under a Creative Commons Attribution-Non Commercial-Share Alike 4.0 International License.

A Gyrovirus (Chicken anemia virus, Gen Bank accession number JX260426) was chosen as an outgroup. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material.

The genomic organization was identical with a Rep ORF interrupted by a 166 nt long intron with a typical splice donor (GT) and acceptor site (AG) and Cap ORF of the same length and positions (Figure 1).

Rep sequence (292 aa) was ≈98% identical to that of Cy CV-VN species.

Vietnamese VN strains are colored in grey, Malawian VS5700009 in light yellow and Tunisian TN25 and TN18 appear in orange Table 2: Sequence identity matrix for the Capsid protein sequence (%).

Cameroonian swine (KM392284–KM392286) and Madagascan human cycloviruses (KM392287–KM392289) described in this study are bolded. All authors were involved in reviewing and editing of the manuscript.

In spite of this, the Rep protein sequence of Cameroonian swine Cy CV-VN-like strains was highly conserved (≈94% identity compared to Madagascan and Vietnamese strains).

Since Cy CVs in the same species are defined as having 85% identity in the Rep protein sequence, these three Cameroonian strains clearly belong to the Cy CV-VN group/species (Figure 2).

Their Rep sequence identity to Malawian VS5700009 and Tunisian TN25 Cy CVs was ≈77% and ≈74%, respectively (Table 1).